Additionally, six genes were uniquely responsive to acutely toxic extracts of OSPW.
Evidence presented supports a role for sulphur- and nitrogen-containing chemical classes in the toxicity of extracts of OSPW.
Using the K-12 strain MG1655 gene reporter live cell array, the present study investigated relationships between toxic potencies of each fraction, expression of genes and characterization of chemicals in each of five acutely toxic and one non-toxic extract of OSPW derived by use of effects-directed analysis.
Effects on expressions of genes related to response to oxidative stress, protein stress and DNA damage were indicative of exposure to acutely toxic extracts of OSPW.
Fluorescence steady-state polarization revealed that the conformation of poliovirus capsid is sensitive to DO effect was demonstrated by the greatly increased value of relative fluorescence intensity.
Conformational alteration was also shown by changes in the positive band (193–230 nm) of circular dichroism spectra.
Although several dehydrogenation pathways have been proposed, the hydrogenation process is less well understood.
Oil sands process-affected water (OSPW) is generated during extraction of bitumen in the surface-mining oil sands industry in Alberta, Canada, and is acutely and chronically toxic to aquatic organisms.
Loss of the 22 γ-Pcdhs, adhesion molecules that interact homophilically and are expressed combinatorially in neurons and astrocytes, leads to aberrantly high activity of focal adhesion kinase (FAK) and reduced dendrite complexity in cortical neurons.Elucidating the underlying mechanism of AGO-mi RNA recognition is thus of great importance not only for the in-depth understanding of mi RNA function but also for inspiring new drugs targeting mi RNAs.In this chapter we introduce a combined computational approach of molecular dynamics (MD) simulations, Markov state models (MSMs), and protein-RNA docking to investigate AGO-mi RNA recognition.In an agricultural context, however, abscission may become a major limiting factor for crop productivity.Domestication of major crops included the selection of plants that did not naturally shed ripe fruits or seeds.